Regulatory

Part:BBa_K1510002:Design

Designed by: Yen-An Chang   Group: iGEM14_NYMU-Taipei   (2014-10-06)

PnlmC (S.mutans)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS.

NYMU yenanchang eletrophoration fo nlmC promoter.jpg

electrophoration of nlmC promoter


Source

Streptococcus mutans UA159

References

Liu, T., et al., ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Annals of Microbiology, 2014. 64(1): p. 31-38.